Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume 103, Issue 12 , Pages 1190-1194, December 2009

FLOTAC: A promising technique for detecting helminth eggs in human faeces

  • Stefanie Knopp

      Affiliations

    • Department of Public Health and Epidemiology, Swiss Tropical Institute, P.O. Box, CH–4002 Basel, Switzerland
    • Helminth Control Laboratory Unguja, Ministry of Health and Social Welfare, P.O. Box 236, Mianzini, Zanzibar, Tanzania
    • Corresponding Author InformationCorresponding author. Tel.: +41 61 284 8226 fax: +41 61 284 8105.
  • ,
  • Dominik Glinz

      Affiliations

    • Department of Public Health and Epidemiology, Swiss Tropical Institute, P.O. Box, CH–4002 Basel, Switzerland
    • Centre Suisse de Recherches Scientifiques, 01 BP 1303, Abidjan 01, Côte d’Ivoire
  • ,
  • Laura Rinaldi

      Affiliations

    • Department of Pathology and Animal Health, CREMOPAR Regione Campania, University of Naples ‘Federico II’, Via della Veterinaria 1, 80137 Naples, Italy
  • ,
  • Khalfan A. Mohammed

      Affiliations

    • Helminth Control Laboratory Unguja, Ministry of Health and Social Welfare, P.O. Box 236, Mianzini, Zanzibar, Tanzania
  • ,
  • Eliézer K. N’Goran

      Affiliations

    • Centre Suisse de Recherches Scientifiques, 01 BP 1303, Abidjan 01, Côte d’Ivoire
    • UFR Biosciences, Université de Cocody-Abidjan, 22 BP 770, Abidjan 22, Côte d’Ivoire
  • ,
  • J. Russell Stothard

      Affiliations

    • Wolfson Wellcome Biomedical Laboratories, Biomedical Parasitology Division, Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UK
  • ,
  • Hanspeter Marti

      Affiliations

    • Department of Medical and Diagnostic Services, Swiss Tropical Institute, P.O. Box, CH–4002 Basel, Switzerland
  • ,
  • Giuseppe Cringoli

      Affiliations

    • Department of Pathology and Animal Health, CREMOPAR Regione Campania, University of Naples ‘Federico II’, Via della Veterinaria 1, 80137 Naples, Italy
  • ,
  • David Rollinson

      Affiliations

    • Wolfson Wellcome Biomedical Laboratories, Biomedical Parasitology Division, Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UK
  • ,
  • Jürg Utzinger

      Affiliations

    • Department of Public Health and Epidemiology, Swiss Tropical Institute, P.O. Box, CH–4002 Basel, Switzerland

Received 19 February 2009; received in revised form 7 May 2009; accepted 7 May 2009.

Summary 

There is a tendency to neglect diagnostic issues in the era of ‘preventive chemotherapy’ in human helminthiases. However, accurate diagnosis cannot be overemphasized for adequate patient management and monitoring of community-based control programmes. Implicit is a diagnostic dilemma: the more effective interventions are in reducing helminth egg excretion, the less sensitive direct parasitological tests become. Here, experiences gained thus far with the FLOTAC technique for diagnosing common soil-transmitted helminth infections are summarized. A single FLOTAC has higher sensitivity than multiple Kato–Katz thick smears in detecting low-intensity infections. Further validation of the FLOTAC technique in different epidemiological settings is warranted, including diagnosis of intestinal schistosomiasis and food-borne trematodiases.

Keywords: Ascaris lumbricoides, Hookworm, Trichuris trichiura, Diagnosis, FLOTAC, Kato–Katz

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 Based on a presentation to the Royal Society of Tropical Medicine & Hygiene Research in Progress meeting on 18 December 2008. This oral presentation was awarded second prize at the meeting.

PII: S0035-9203(09)00196-5

doi:10.1016/j.trstmh.2009.05.012

Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume 103, Issue 12 , Pages 1190-1194, December 2009