Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume 99, Issue 12 , Pages 893-900 , December 2005

Detection of mycobacterial lipoarabinomannan with an antigen-capture ELISA in unprocessed urine of Tanzanian patients with suspected tuberculosis

  • C. Boehme

      Affiliations

    • Department of Infectious Diseases and Tropical Medicine, LMU, University of Munich, Leopoldstr. 5, 80802 Munich, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49 89 21803517; fax: +49 89 336112.
    • ,
  • E. Molokova

      Affiliations

    • Chemogen, Inc., Chemogen, So. Portland, Maine, USA
    • ,
  • F. Minja

      Affiliations

    • Regional Medical Office, Tanzanian Ministry of Health, Mbeya, Tanzania
    • ,
  • S. Geis

      Affiliations

    • Department of Infectious Diseases and Tropical Medicine, LMU, University of Munich, Leopoldstr. 5, 80802 Munich, Germany
    • ,
  • T. Loscher

      Affiliations

    • Department of Infectious Diseases and Tropical Medicine, LMU, University of Munich, Leopoldstr. 5, 80802 Munich, Germany
    • ,
  • L. Maboko

      Affiliations

    • Mbeya Medical Research Programme, Mbeya, Tanzania
    • ,
  • V. Koulchin

      Affiliations

    • Chemogen, Inc., Chemogen, So. Portland, Maine, USA
    • ,
  • M. Hoelscher

      Affiliations

    • Department of Infectious Diseases and Tropical Medicine, LMU, University of Munich, Leopoldstr. 5, 80802 Munich, Germany

Received 18 November 2004 ,Revised 8 April 2005 ,Accepted 12 April 2005.

References 

  1. Apers L, Mutsvangwa J, Magwenzi J, Chigara N, Butterworth A, Mason P, et al. A comparison of direct microscopy, the concentration method and the Mycobacteria Growth Indicator Tube for the examination of sputum for acid-fast bacilli. Int. J. Tuberc. Lung Dis. 2003;7:376–381
  2. Boggian K, Fierz W, Vernazza PL. Infrequent detection of lipoarabinomannan antibodies in human immunodeficiency virus-associated mycobacterial disease. Swiss HIV Cohort Study. J. Clin. Microbiol. 1996;34:1854–1855
  3. Burman WJ, Jones BE. Clinical and radiographic features of HIV-related tuberculosis. Semin. Respir. Infect. 2003;18:263–271
  4. Chan J, Fan XD, Hunter SW, Brennan PJ, Bloom BR. Lipoarabinomannan, a possible virulence factor involved in persistence of Mycobacterium tuberculosis within macrophages. Infect. Immun. 1991;59:1755–1761
  5. Daffe M, Draper P. The envelope layers of mycobacteria with reference to their pathogenicity. Adv. Microb. Physiol. 1998;39:131–203
  6. Doherty TM, Demissie A, Olobo J, Wolday D, Britton S, Eguale T, et al. Immune responses to the Mycobacterium tuberculosis-specific antigen ESAT-6 signal subclinical infection among contacts of tuberculosis patients. J. Clin. Microbiol. 2002;40:704–706
  7. Doskeland SO, Berdal BP. Bacterial antigen detection in body fluids: methods for rapid antigen concentration and reduction of nonspecific reactions. J. Clin. Microbiol. 1980;11:380–384
  8. Githui W, Kitui F, Juma ES, Obwana DO, Mwai J, Kwamanga D. A comparative study on the reliability of the fluorescence microscopy and Ziehl-Neelsen method in the diagnosis of pulmonary tuberculosis. East Afr. Med. J. 1993;70:263–266
  9. Hamasur B, Bruchfeld J, Haile M, Pawlowski A, Bjorvatn B, Kallenius G, et al. Rapid diagnosis of tuberculosis by detection of mycobacterial lipoarabinomannan in urine. J. Microbiol. Methods. 2001;45:41–52
  10. Hunter SW, Gaylord H, Brennan PJ. Structure and antigenicity of the phosphorylated lipopolysaccharide antigens from the leprosy and tubercle bacilli. J. Biol. Chem. 1986;261:12345–12351
  11. Johnson PD, Stuart RL, Grayson ML, Olden D, Clancy A, Ravn P, et al. Tuberculin-purified protein derivative-, MPT-64-, and ESAT-6-stimulated gamma interferon responses in medical students before and after Mycobacterium bovis BCG vaccination and in patients with tuberculosis. Clin. Diagn. Lab. Immunol. 1999;6:934–937
  12. Lee RE, Brennan PJ, Besra GS. Mycobacterium tuberculosis cell envelope. Curr. Top. Microbiol. Immunol. 1996;215:1–27
  13. Levy H, Feldman C, Sacho H, van der Meulen H, Kallenbach J, Koornhof H. A reevaluation of sputum microscopy and culture in the diagnosis of pulmonary tuberculosis. Chest. 1989;95:1193–1197
  14. Ong A, Creasman J, Hopewell PC, Gonzalez LC, Wong M, Jasmer RM, et al. A molecular epidemiological assessment of extrapulmonary tuberculosis in San Francisco. Clin. Infect. Dis. 2004;38:25–31
  15. Pereira Arias-Bouda LM, Nguyen LN, Ho LM, Kuijper S, Jansen HM, Kolk AH. Development of antigen detection assay for diagnosis of tuberculosis using sputum samples. J. Clin. Microbiol. 2000;38:2278–2283
  16. Pollock JM, McNair J, Bassett H, Cassidy JP, Costello E, Aggerbeck H, et al. Specific delayed-type hypersensitivity responses to ESAT-6 identify tuberculosis-infected cattle. J. Clin. Microbiol. 2003;41:1856–1860
  17. Pottumarthy S, Wells VC, Morris AJ. A comparison of seven tests for serological diagnosis of tuberculosis. J. Clin. Microbiol. 2000;38:2227–2231
  18. Ratanasuwan W, Kreiss JK, Nolan CM, Schaeffler BA, Suwanagool S, Tunsupasawasdikul S, et al. Evaluation of the MycoDot test for the diagnosis of tuberculosis in HIV seropositive and seronegative patients. Int. J. Tuberc. Lung Dis. 1997;1:259–264
  19. Ravn P, Demissie A, Eguale T, Wondwosson H, Lein D, Amoudy HA, et al. Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis. J. Infect. Dis. 1999;179:637–645
  20. Sada E, Brennan PJ, Herrera T, Torres M. Evaluation of lipoarabinomannan for the serological diagnosis of tuberculosis. J. Clin. Microbiol. 1990;28:2587–2590
  21. Sada E, Aguilar D, Torres M, Herrera T. Detection of lipoarabinomannan as a diagnostic test for tuberculosis. J. Clin. Microbiol. 1992;30:2415–2418
  22. Tessema TA, Hamasur B, Bjun G, Svenson S, Bjorvatn B. Diagnostic evaluation of urinary lipoarabinomannan at an Ethiopian tuberculosis centre. Scand. J. Infect. Dis. 2001;33:279–284
  23. Tessema TA, Bjune G, Assefa G, Svenson S, Hamasur B, Bjorvatn B. Clinical and radiological features in relation to urinary excretion of lipoarabinomannan in Ethiopian tuberculosis patients. Scand. J. Infect. Dis. 2002;34:167–171
  24. Tessema TA, Bjune G, Hamasur B, Svenson S, Syre H, Bjorvatn B. Circulating antibodies to lipoarabinomannan in relation to sputum microscopy, clinical features and urinary anti-lipoarabinomannan detection in pulmonary tuberculosis. Scand. J. Infect. Dis. 2002;34:97–103
  25. Ulrichs T, Munk ME, Mollenkopf H, Behr-Perst S, Colangeli R, Gennaro ML, et al. Differential T cell responses to Mycobacterium tuberculosis ESAT6 in tuberculosis patients and healthy donors. Eur. J. Immunol. 1998;28:3949–3958
  26. Yang Z, Kong Y, Wilson F, Foxman B, Fowler AH, Marrs CF, et al. Identification of risk factors for extrapulmonary tuberculosis. Clin. Infect. Dis. 2004;38:199–205

PII: S0035-9203(05)00173-2

doi: 10.1016/j.trstmh.2005.04.014

Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume 99, Issue 12 , Pages 893-900 , December 2005

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