Volume 96, Supplement 1 , Pages S75-S80, April 2002
Homologous minicircles in Leishmania donovani☆
Abstract
Leishmania minicircular deoxyribonucleic acid (DNA) is arranged into different classes according to sequence. These classes differ substantially in sequence, despite species- and genusspecific regions, and are present in widely different copy numbers within and between Leishmania strains. Homologous minicircles have been identified in different species of Leishmania by comparing sequences of known minicircles. However, it is possible to select for minicircles of the same class by amplifying Leishmania DNA with polymerase chain reaction primers from the conserved and variable regions. This approach was used with 2 different minicircle classes in the L. donovani complex. In all isolates tested it was possible to amplify minicircles of the selected class.
Keywords: leishmaniasis, Leishmania donovani, DNA, kinetoplast DNA, minicircle class, sequencing kDNA
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☆ Nucleotide sequence data reported in this paper are available in the EMBL, GenBank™ and DDJB databases with the accession numbers AF168356 (RTC6), AF190476 (MARZ-KRIM), AF167712 (DD8), AF167715 (TANGAIL), AF167718 (L57), AF167717 (MRC74), AF184892 (H9) for the Gutiérrez-Solar minicircles and AF239704 (GG996), AF239702 (H9), AF239703 (MRC74) for the ND8 minicircles.
PII: S0035-9203(02)90055-6
© 2002 Published by Elsevier Inc.
Volume 96, Supplement 1 , Pages S75-S80, April 2002
