Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume 96, Supplement 1 , Pages S71-S73, April 2002

A kinetoplast DNA minicircle probe (B18) specific for the Leishmania subgenus Viannia

  • L.J. Gibson

      Affiliations

    • Imperial College School of Medicine, Charing Cross Hospital, London, UK
    • ,
  • S.H.E. McCann

      Affiliations

    • Molteno Laboratories, Department of Pathology, University of Cambridge, Cambridge, UK
    • Present address and address for correspondence: S. H. E. McCann, Wellcome Trust Centre for Molecular Mechanisms in Disease, Wellcome/MRC Building, CIMR, Hills Road, Cambridge, CB2 2XY, UK.
    • ,
  • S. Brewster

      Affiliations

    • Molteno Laboratories, Department of Pathology, University of Cambridge, Cambridge, UK
    • ,
  • D.C. Barker

      Affiliations

    • Molteno Laboratories, Department of Pathology, University of Cambridge, Cambridge, UK

Abstract 

This paper reports the development, sequence and specificity of probe B18, which hybridizes only to the kinetoplast deoxyribonucleic acid (kDNA) minicircle of Leishmania species of the subgenus Viannia. This probe was developed in 1985 and has been used extensively since, on whole kDNA, Leishmania dot-blots and kDNA minicircles amplified by the polymerase chain reaction.

Keywords:  leishmaniasis, Leishmania (Viannia) spp., diagnosis, kinetoplast DNA minicircles, probe B18

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PII: S0035-9203(02)90054-4

Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume 96, Supplement 1 , Pages S71-S73, April 2002

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