Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume 96 , Pages S41-S47 , April 2002

Isolation and characterization of Leishmania infantum cDNA encoding a protein homologous to eukaryotic elongation factor 1γ

  • Carmen Cañavate

      Affiliations

    • Corresponding Author InformationAddress for correspondence: Carmen Cañavate, WHO Collaborating Centre for Leishmaniasis, Servicio de Parasitoloía, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo km. 2, 28220 Majadahonda, Madrid, Spain; phone +34 91 5097901, fax +34 91 5097034.
    • Instituto de Salud Carlos III, Centro Nacional de Microbiología, Servicio de Parasitología, WHO Collaborating Centre for Leishmaniasis, Madrid, Spain
    • ,
  • Mohammed El Bali

      Affiliations

    • Instituto de Salud Carlos III, Centro Nacional de Microbiología, Servicio de Parasitología, WHO Collaborating Centre for Leishmaniasis, Madrid, Spain
    • ,
  • Ysabel Montoya

      Affiliations

    • Instituto Nacional de Salud, Capac Yupanqui Lima, Perú
    • ,
  • Douglas C. Barker

      Affiliations

    • Molteno Laboratories, Department of Pathology, University of Cambridge, Cambridge, UK
    • ,
  • Jorge Alvar

      Affiliations

    • Instituto de Salud Carlos III, Centro Nacional de Microbiología, Servicio de Parasitología, WHO Collaborating Centre for Leishmaniasis, Madrid, Spain

References 

  1. Alvar J, Cañavate C, Gutiérrez-Solar B, Jiménez M, Laguna F, López-Vélez R, et al. Leishmania and human immunodeficiency virus coinfection: the first 10 years. Clinical Microbiology Reviews. 1997;10:298–319
  2. Bastien P, Blaineau C, Pagès M. Molecular karyotype analysis in Leishmania. In: Intracellular Parasites.  Avila JL,  Harris R editor. Subcellular Biochemistry. 18:New York: Plenum Press; 1992;p. 131–187
  3. Billaut-Mulot O, Pommier V, Schöneck R, Plumas-Marty B, Taibi A, Loyens M, et al. Nucleotide sequence of a Trypanosoma cruzi cDNA encoding a protein homologous to mammalian EF-1γ. Nucleic Acids Research. 1993;21:3901
  4. Billaut-Mulot O, Schöneck R, Fernandez-Gomez R, Taibi A, Capron A, Pommier V, et al. Molecular and immunological characterization of a Trypanosoma cruzi protein homologous to mammalian elongation factor 1 gamma. Biology of the Cell. 1994;82:39–44
  5. Billaut-Mulot O, Fernandez-Gomez R, Ouaissi A. Phenotype of recombinant Trypanosoma cruzi which over-expresses elongation factor 1-γ: possible involvement of EF-1γ GST-like domain in the resistance to clomipramine. Gene. 1997;198:259–267
  6. Blin N, Stafford DW. A general method for the isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Research. 1976;3:2303–2308
  7. Cornier P, Osborne HB, Morales J, Bassez T, Poulhe R, Mulner-Lorillon O, et al. Molecular cloning of Xenopus elongation factor 1γ, major M-phase promoting factor substrate. Nucleic Acids Research. 1991;19:6644
  8. Desjeux P. Human leishmaniases: epidemiology and public health aspects. World Health Statistics Quarterly. 1992;45:267–275
  9. Howard JG. Host immunity to leishmaniasis. In:  Chang KP,  Bray RS editor. Leishmaniasis. Amsterdam: Elsevier; 1985;p. 139–162
  10. Hyunh TV, Young RA, Davis RW. In:  Glover DM editors. DNA Cloning. A Practical Approach. vol. 1:Oxford: IRL Press; 1985;p. 49–78
  11. Janssen GMC, Möller W. Kinetic studies on the role of elongation factors 1β and 1γ in protein synthesis. Journal of Biological Chemistry. 1988;263:1773–1778
  12. Janssen GMC, Möller W. Elongation factor 1βγ from Artemia. Purification and properties of its subunits. European Journal of Biochemistry. 1988;171:119–129
  13. Janssen GMC, Morales J, Schipper A, Labbé JC, Mulner-Lorillon O, Bellé R, et al. A major substrate of maturation promoting factor identified as elongation factor 1βγδ in Xenopus laevis. Journal of Biological Chemistry. 1991;266:14885–14888
  14. Kidou S, Tsukamoto S, Kobayashi S, Ejiri S. Isolation and characterization of a rice cDNA encoding the γ-subunit of translation elongation factor 1 B (eEF1Bγ). FEBS Letters. 1998;434:382–386
  15. Koonin EV, Mushegian AR, Tatusov RL, Altschul SF, Bryant SH, Bork P, et al. Eukaryotic translation elongation factor 1γ contains a glutathione transferase domain—study of a diverse, ancient protein super-family using motif search and structural modeling. Protein Science. 1994;3:2045–2054
  16. Kozac M. Comparison of initiation of protein synthesis in prokaryotes, eukaryotes and organelles. Microbiological Reviews. 1983;47:1–45
  17. Kumabe T, Sohma Y, Yamamoto T. Human cDNAs encoding elongation factor 1γ and the ribosomal protein L19. Nucleic Acids Research. 1992;20:2598
  18. Ladeira de Campos CB, Lopes UG. Leishmania braziliensis, molecular characterization of an elongation factor 1α gene. Gene. 1997;198:281–288
  19. LeBowitz JH, Smith HQ, Rusche I, Beverly SM. Coupling of poly(A) site selection and trans-splicing in Leishmania. Genes and Development. 1993;7:996–1007
  20. Merrick WC. Mechanism and regulation of eukaryotic protein synthesis. Microbiological Reviews. 1992;56:291–315
  21. Mimori K, Mori M, Tanaka S, Akiyoshi T, Sugimachi K. The overexpression of elongation factor-1 gamma mRNA in gastric carcinoma. Cancer. 1995;75:1446–1449
  22. Rangel-Aldao R, Comach G, Mendoza A. In vitro translation of T. cruzi antigens recognized by human chagasic sera. Journal of Parasitology. 1987;73:855–857
  23. Requena JM, Alonso C, Soto M. Evolutionarily conserved proteins as prominent immunogens during Leishmania infections. Parasitology Today. 2000;16:246–250
  24. Sambrook J, Fritsch EF, Maniatis T. In: 2nd edition. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989;
  25. Sanders J, Brandsma M, Janssen GMC, Dijk J, Möller W. Immunofluorescence studies of human fibroblasts demonstrate the presence of the complex of elongation factor-1βγδ in the endoplasmic reticulum. Journal of Cell Science. 1996;109:1113–1117
  26. Sheu GT, Traugh JA. Nucleotide sequence of a rabbit cDNA encoding elongation factor 1 gamma. Nucleic Acids Research. 1992;20:5849
  27. Stiles JK, Hicock PI, Shah PH, Meade JC. Genomic organization, transcription, splicing and gene regulation in Leishmania. Annals of Tropical Medicine and Parasitology. 1999;93:781–807
  28. Wilson K, Hanson S, Landfear S, Ullman B. Nucleotide sequence of the Leishmania donovani medRNA gene. Nucleic Acids Research. 1991;19:5787
  29. Wincker P, Ravel C, Blaineau C, Pagès C, Jaufret Y, Dedet J-P, et al. The Leishmania genome comprises 36 chromosomes conserved across widely divergent human pathogenic species. Nucleic Acids Research. 1996;24:1688–1694

PII: S0035-9203(02)90050-7

Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume 96 , Pages S41-S47 , April 2002

Article Tools