Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume 96, Supplement 1 , Pages S41-S47, April 2002

Isolation and characterization of Leishmania infantum cDNA encoding a protein homologous to eukaryotic elongation factor 1γ

  • Carmen Cañavate

      Affiliations

    • Corresponding Author InformationAddress for correspondence: Carmen Cañavate, WHO Collaborating Centre for Leishmaniasis, Servicio de Parasitoloía, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo km. 2, 28220 Majadahonda, Madrid, Spain; phone +34 91 5097901, fax +34 91 5097034.
    • Instituto de Salud Carlos III, Centro Nacional de Microbiología, Servicio de Parasitología, WHO Collaborating Centre for Leishmaniasis, Madrid, Spain
    • ,
  • Mohammed El Bali

      Affiliations

    • Instituto de Salud Carlos III, Centro Nacional de Microbiología, Servicio de Parasitología, WHO Collaborating Centre for Leishmaniasis, Madrid, Spain
    • ,
  • Ysabel Montoya

      Affiliations

    • Instituto Nacional de Salud, Capac Yupanqui Lima, Perú
    • ,
  • Douglas C. Barker

      Affiliations

    • Molteno Laboratories, Department of Pathology, University of Cambridge, Cambridge, UK
    • ,
  • Jorge Alvar

      Affiliations

    • Instituto de Salud Carlos III, Centro Nacional de Microbiología, Servicio de Parasitología, WHO Collaborating Centre for Leishmaniasis, Madrid, Spain

Abstract 

This paper reports the isolation and characterization of a complementary deoxyribonucleic acid clone showing sequence homology with genes coding for the eukaryotic elongation factor 1γ (EF-1γ). The clone encodes an open reading frame of 404 amino acids corresponding to a deduced molecular mass of 46·2 kDa. Database searches revealed 30–64% sequence identity between the Leishmania infantum EF-1γ and several eukaryotic homologues. Southern blot analysis indicated that 2 genes tandemly organized were present in the L. infantum genome. The 3′ untranslated regions of these 2 genes differed in size. Southern hybridization and pulsed field gel electrophoresis showed that EF-1γ genes are highly conserved among members of the Leishmania genus and must be clustered in a single chromosomal locus.

Keywords:  leishmaniasis, Leishmania infantum, genomic characterization, eukaryotic elongation factor (EF) 1γ gene

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PII: S0035-9203(02)90050-7

Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume 96, Supplement 1 , Pages S41-S47, April 2002

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